In Vitro Regeneration and Synthesis of Silver Nanoparticles using Extract of Azadirachta Indica Callus and Their Antimicrobial Activity

Because of Plant Tissue Culture, plants are being regenerated in vitro in sterile containers which greatly reduced chances of transmitting diseases, pests, and pathogens. This all has been possible due to the ability of the plant cells to regenerate a whole plant called tot potency. Any part of the plant can be used to produce medicinally important compounds in vitro. The Azadirachta indica has medically important secondary metabolites that are produced by calls culture using Plant Tissue Culture techniques, leaves as explants. Thus such produced secondary metabolites are used to synthesize silver nanoparticles which showed maximum absorbance at 410nm under U.V.-Visible spectroscope. When these nanoparticles were tested against bacteria Bacillus subtilis and Staphylococcus aureus, B. subtilis showed resistance while S. aureus showed sensitivity with zone of inhibition of 6mm.