Callus Induction and Shoot Regeneration in VIGNA RADIATA

Plant Tissue Culture is a practice used to propagate plants under sterile conditions, often to produce clones of a plant. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation. We have taken the Vigna radiata seeds as explant for callus induction and shoot regeneration. Because Mungbean is a food grain, legume crop all over the world. This crop is regarded as a quality pulse in India for its excellent protein and high digestibility. Several biotic and abiotic factors as well as low genetic variability are supposed to be responsible for low production of this crop. Explant was sterilized and inoculated on callus induction and shoot regeneration medium separately supplemented with hormones. The medium used for callus induction includes MS medium and other hormones like 2,4-D and Kinetin and medium used for shoot regeneration includes MS medium and other hormones like Kinetin and BAP and the explants were incubated in tissue culture lab under aseptic conditions and light and temperature of 25 ± 20C was provided. After first week, discolorations of explants were observed, after 3 weeks small proliferations appeared on the explant surface. The undifferentiated mass of cells i.e. callus is developed after 5 weeks. In shoot regeneration culture tubes after 2 weeks leaf primordia was observed, and the differentiation and elongation of shoots were observed during 6 weeks.